Gene and Its Engineering
ISBN: 9788126549283
588 pages
For more information write to us at: acadmktg@wiley.com
Description
The book covers molecular biology of the gene and genetic engineering. It is an endeavour to expose students to the actual experiments that have revealed the science of gene and the development of its engineering. The book provides comprehensive details and chronological evolution of the structure of gene, its replication and expression, and genetic engineering based on significant discoveries made by the scientists. It is a comprehensive source of information to both students and instructors.
Part I. Gene, its replication and its expression
1 Concept of a gene
2 DNA as the genetic material
3 Constituents of nucleic acids
4 Structure of DNA
5 Characteristics of DNA
6 Chromatin and its structure
7 Chromatin remodelling
8 Classic experiments on DNA replication in vivo
9 DNA polymerases
10 Mechanics of DNA replication
11 RNA, its transcription and processing
12 How transcription of genes is switched on and off
13 Structure and replication of RNA
14 Translation of mRNA into protein: Classic and recent experiments
15 Translation regulation of gene expression and human diseases
16 Non-coding RNA
Part II. Engineering of Gene
17 Cloning of gene: How it all began
18 Reaction of the scientific community after successful cloning of a gene
19 Genetic engineering guidelines
20 Tools used in genetic engineering
21 General strategies for expression of introduced genes in a foreign host
22 Gram-negative bacterium Escherichia coli as host for recombinant DNA
23 Gram-positive bacterium Bacillus subtilis as host for recombinant DNA
24 Streptomyces as host for recombinant DNA
25 Yeast Pichia pastoris as host for recombinant DNA
26 Insect cells as host for recombinant DNA
27 Animal cells as host for recombinant DNA
28 Plants as host for recombinant DNA
29 Chemical and enzymatic characterization of a cloned DNA fragment
30 Reverse transcription and cDNA cloning
31 Nucleic acid amplification and its applications
32 Alternative strategies of gene cloning
33 Site-directed In Vitro mutagenesis and protein engineering
34 Processing of recombinant proteins
35 Techniques for the study of regulation of a cloned gene
36 Transgenic and gene knockout technologies
37 Gene therapy
Colour plates
Index
Appendix I
Appendix II