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Biotechnology for All

Tarun Rajpal, Rajni Gupta

ISBN: 9789363867147

444 pages

INR 499

For more information write to us at: acadmktg@wiley.com

Description

Biotechnology for All – Your Gateway to Understanding Biotechnology

"Biotechnology for All" is a comprehensive and student-friendly book designed to simplify the complex world of biotechnology. Perfect for beginners, this book bridges the gap between fundamental biological concepts and their applications in biotechnology.

Tailored to meet the needs of students, it covers key topics in biotechnology with precision and clarity, making it an excellent resource for exam preparation. The content is presented in a crisp and concise format to ensure conceptual clarity and an engaging learning experience. Whether you're new to the subject or looking to strengthen your basics, "Biotechnology for All" is the ultimate guide to mastering the essentials of biotechnology.

1. INTRODUCTION

1.1 Definitions of Biotechnology

1.2 Old and New Biotechnology

1.3 Scope of Biotechnology

1.4 Biotechnology in India

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2. NUCLEIC ACIDS

2.1 Introduction

2.2 Composition of Nucleic Acids

2.3 Difference Between Nucleotide and Nucleoside

2.4 Structural Orientation of Nucleotide

2.5 Abbreviation Codes for Degenerate Bases of Nucleotides

2.6 Molecular Structures

2.7 Forms of DNA

2.8 Forms of RNA

2.9 Difference Between DNA and RNA

2.10 Molecular Structures

2.11 Transposons

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3. AMINO ACIDS

3.1 Introduction

3.2 General Structure

3.3 Classifi cation of Amino Acids

3.4 Amino Acids for Human Body

3.5 Zwitterions

3.6 Proteins

3.7 Proteomics

3.8 Protein Engineering

3.9 Sickle-Cell Anemia

3.10 Immunotoxins

3.11 Drug Designing

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4. rDNA TECHNOLOGY

4.1 Introduction

4.2 Basic Steps of rDNA Technology

4.3 Restriction Enzymes

4.4 Other Enzymes

4.5 Frequency of Restriction/Recognition Sequence in a DNA Molecule

4.6 Restriction Ends

4.7 Vectors

4.8 Host Cells

4.9 DNA Library

4.10 Introduction of rDNA Molecule into Host Cell

4.11 Identification of Recombinants

4.12 Selection of Clone Containing Specific DNA Insert

4.13 Primer Walking

4.14 Chromosome Walking

4.15 Chromosome Jumping

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5. BASIC TECHNIQUES

5.1 Electrophoresis

5.2 Polymerase Chain Reaction

5.3 Autoradiography

5.4 Southern Hybridization

5.5 Northern Hybridization

5.6 Western Blotting

5.7 DNA Sequencing

5.8 DNA Fingerprinting

5.9 DNA Microarray

5.10 Site-Directed Mutagenesis

5.11 Enzyme-Linked Immunosorbent Assay

5.12 Nick Translation and Fluorescence In Situ Hybridization

5.13 Mass Spectrometry

5.14 Gene Therapy

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6. IMMUNOLOGY

6.1 Introduction

6.2 Types of Immunity

6.3 Cells of Immune System

6.4 Organs of Immune System

6.5 Antigens

6.6 Superantigens

6.7 Antibodies

6.8 Major Histocompatibilty Complex

6.9 Immunological Tolerance

6.10 Hypersensitivity

6.11 Autoimmunity

6.12 Vaccines

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7. GENOMICS

7.1 Introduction

7.2 Types of Genomics

7.3 Genome Sequencing Projects

7.4 Single-Nucleotide Polymorphism

7.5 Genetic Maps

7.6 Genetic Marker

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8. BIOINFORMATICS

8.1 Introduction

8.2 Biological Data

8.3 Aim of Bioinformatics

8.4 Scope of Bioinformatics

8.5 Bioinformatics in India

8.6 Branches of Bioinformatics

8.7 Database

8.8 Biological Databases

8.9 Homologous Genes

8.10 National Center for Biotechnology Information

8.11 European Molecular Biology Laboratory Nucleotide Sequence Database

8.12 DNA Data Bank of Japan

8.13 Protein Information Resource

8.14 Swiss-Prot

8.15 Point Accepted Mutation

8.16 Blocks of Amino Acid Substitution Matrix (BLOSUM)

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9. PHYLOGENETIC ANALYSIS

9.1 Introduction

9.2 Types of Phylogenetic Tree

9.3 Dendrogram, Cladogram, and Phylogram

9.4 Monophyletic, Paraphyletic, and Polyphyletic Groups

9.5 Molecular Clock Theory

9.6 Number of Rooted and Unrooted Trees

9.7 Methods for Inferring Molecular Phylogenies

9.8 Methods for Cladogram Robustness

9.9 Software Programs Available for Phylogenetic Analysis

9.10 FASTA Format

9.11 Sequence Alignment

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10. PLANT TISSUE CULTURE

10.1 Introduction

10.2 Basic Steps Involved in Plant Tissue Culture

10.3 Nutrient Media

10.4 Types of In Vitro Cultures

10.5 Methods for Isolation of Single Cells

10.6 Single-Cell Culture Techniques

10.7 Cytodifferentiation

10.8 Plant Regeneration Pathways

10.9 Somaclonal Variation

10.10 In vitro Germplasm Conservation

10.11 Gene Transfer Methods in Plants

10.12 Protoplast Fusion

10.13 Plants with Benefi cial Traits

10.14 Delayed Fruit Ripening

10.15 Male Sterility

10.16 Golden Rice

10.17 Biodegradable Plastic

10.18 Edible Vaccines

10.19 Applications of Plant Tissue Culture

10.20 Molecular Pharming

10.21 Bioethics

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11. ANIMAL TISSUE CULTURE

11.1 Introduction

11.2 Advantages of Animal Tissue Culture

11.3 Disadvantages of Animal Tissue Culture

11.4 Contact Inhibition

11.5 Differences Between In Vitro and In Vivo Cell Growths

11.6 Organ Culture

11.7 Cell Culture

11.8 Differences Between Organ Culture and Cell Culture

11.9 Immobilized Cell Cultures

11.10 Gas Phase

11.11 Hayflick Limit

11.12 Culture Media

11.13 Environmental Factors

11.14 Equipments for Animal Tissue Culture

11.15 Preservation Methods

11.16 Transgenic Animals

11.17 Interferon

11.18 Somatic Cell Fusion

11.19 Hybridoma Technology

11.20 Monoclonal Antibodies

11.21 Embryonic Stem Cells Transfer

11.22 In Vitro Fertilization and Embryo Transfer in Humans

11.23 Products of Animal Tissue Culture

11.24 Bioethics

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12. MICROBIAL CELL CULTURE

12.1 Introduction

12.2 Bacterial Growth Curve

12.3 Requirements for Microbial Cell Culture

12.4 Factors Affecting Growth of Microorganisms

12.5 Types of Microbial Cultures

12.6 Downstream Processing

12.7 Strain Isolation

12.8 Strain Improvement

12.9 Methods for Strain Preservation

12.10 Bioethics

12.11 Enzymes (Introduction)

12.12 Classification of Enzymes

12.13 Enzymes and Non-Biological Catalysts

12.14 Fermentation

12.15 Production of Enzymes by Microorganisms

12.16 Beer Production

12.17 Vinegar Production

12.18 Cheese Production

12.19 Uses of Enzymes

12.20 Role of Microorganisms

12.21 Microbial Transformations

12.22 Single-Cell Protein

12.23 Microbial Biomass

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13. ENVIRONMENTAL BIOTECHNOLOGY

13.1 Introduction

13.2 Renewable and Non-Renewable Sources of Energy

13.3 Biofuel

13.4 Bioremediation

13.5 Biostimulation

13.6 Bioaugmentation

13.7 Vermicomposting

13.8 Bioleaching

13.9 Biosensors

13.10 Biodegradable Plastics

13.11 Microbiological Treatment of Municipal Waste and Industrial Effluents

13.12 Biocontrol Agents

13.13 Biomagnifi cation

13.14 Biodesulfurization

13.15 Biosurfactants

13.16 Biopolymers

13.17 Restoration of Degraded Lands

13.18 Biodiversity

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14. BIOSAFETY AND INTELLECTUAL PROPERTY RIGHTS

14.1 Introduction (Biosafety)

14.2 Landmarks in Biosafety

14.3 Objectives of Biosafety Guidelines

14.4 Containment

14.5 Planned Introduction of Genetically Modified Organisms (GMOs)

14.6 Risk Assessment

14.7 Risk Regulation

14.8 Biosafety Guidelines

14.9 Biosafety Guidelines in India

14.10 Introduction (Intellectual Property Rights)

14.11 Forms of Intellectual Property Rights (IPR)

14.12 Patents in Biotechnology

14.13 Protection of Plant Varieties and Animal Breeds

14.14 Trade-Related Intellectual Property Rights (TRIPs)

14.15 Factors Influencing Choice of Intellectual Property Rights (IPR)

14.16 Plant Breeders’ Rights

14.17 Protection of Intellectual Property Rights (IPR) in India

14.18 Case Studies on Intellectual Property Rights (IPR)

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