Principles of Gene Manipulation and Genomics, 7ed (An Indian Adaptation)

Sandy B. Primrose, Richard Twyman, Dr. Aditya Kumar, Dr. Jayendra Nath Shukla, Dr. Shashi Chaudhary

ISBN: 9789354641534

712 pages

Exclusively distributed by CBS Publishers & Distributors

 

Description

This Indian Adaptation of the seventh edition of the book contains updated and extended coverage on Far-Western blotting, Electroporation, PCR, Joining of DNA molecules, Primary nucleotide sequence database, Genetic manipulation of animals, RNA interference, Multidimensional liquid chromatography, Protein structures, etc. A number of new topics have been added to make the coverage of content complete as per the Indian curriculum requirement.

1 Gene Manipulation in the Post-Genomics Era

 

Part I Applications of Recombinant DNA Technology

 

2 Basic Techniques for Isolation of Genetic Materials

3 Recombinant DNA Technology

4 Basic Biology of Plasmid and Phage Vectors

5 Cosmids, Phasmids, and Other Advanced Vectors

6 Gene-Cloning Strategies

7 Sequencing Genes and Short Stretches of DNA

8 Changing Genes: Site-directed Mutagenesis and Protein Engineering

9 Bioinformatics

 

Part II Manipulating DNA in Microbes, Plants, and Animals

 

10 Cloning in Bacteria Other than Escherichia coli Saccharomyces cerevisiae and Other Fungi

11 Gene Transfer to Animal Cells

12 Genetic Manipulation of Animals

13 Gene Transfer to Plants

14 Advanced Transgenic Technology

15 The Organization and Structure of Genomes

16 Mapping and Sequencing Genomes

17 Comparative Genomics

18 Analysis of the Transcriptome

19 Proteomics I – Expression Analysis and Characterization of Proteins

20 Proteomics II – Analysis of Protein Structures

21 Proteomics III – Protein Interactions

22 Metabolomics and Global Biochemical Networks

23 Applications of Pharmacogenomics and Regulations for RDT

24 Applications of Recombinant DNA Technology

 

Appendix 1: The Genetic Code and Single-Letter Amino Acid Designations

Appendix 2: Safety Guidelines* of Recombinant DNA Technology

Appendix 3: Risk Assessment of Microorganisms

 

 

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